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Author (up) Yamane, T.; Miyatake, T.; Kimura, Y. url  doi
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  Title Female mating receptivity after injection of male-derived extracts in Callosobruchus maculatus Type Journal Article
  Year 2008 Publication Journal of Insect Physiology Abbreviated Journal J Insect Physiol  
  Volume 54 Issue 12 Pages 1522-1527  
  Keywords Animals; Beetles/*drug effects/*physiology; Female; Genitalia, Male/anatomy & histology/chemistry; Male; Sexual Behavior, Animal/*drug effects  
  Abstract The effects of male-derived extracts on female receptivity were investigated in Callosobruchus maculatus (Coleoptera: Bruchidae). Injection of aqueous extracts of the male reproductive tract into the abdomen of females reduced receptivity. Aqueous extracts of male reproductive tracts were divided to three molecular weight (MW) fractions by ultrafiltration: Fractions: (I) MW<3 kDa, (II) 3-14 kDa, and (III)>14 kDa. Fraction II reduced female receptivity from 3h after injection, and Fraction III reduced female receptivity from 2 days after injection. On the other hand, no effect on receptivity was found for Fraction I. Furthermore, male reproductive tract organs were divided into accessory gland, testis, and seminal vesicle including the ejaculatory duct. Aqueous extracts of the seminal vesicle reduced receptivity of females immediately following injection, while aqueous extracts of the accessory gland reduced receptivity at the second day. The results suggest that the components of Fraction II existed in the seminal vesicle, and those of Fraction III in the accessory gland. The results of the present and the previous studies in Callosobruchus chinensis, a species closely related to C. maculatus, were compared and are discussed from the viewpoint of the significance of ejaculation in the two species.  
  Address Laboratory of Evolutionary Ecology, Graduate School of Environmental Science, Okayama University, Tsushima-naka 1-1-1, Okayama 700-8530, Japan  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0022-1910 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:18831977 Approved no  
  Call Number Clayton @ BarbaraMusolf @ Serial 39  
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