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Author (up) Zhu-Salzman, K.; Koiwa, H.; Salzman, R.A.; Shade, R.E.; Ahn, J.E. doi  openurl
  Title Cowpea bruchid Callosobruchus maculatus uses a three-component strategy to overcome a plant defensive cysteine protease inhibitor Type Journal Article
  Year 2003 Publication Insect Molecular Biology Abbreviated Journal Insect Mol Biol.  
  Volume 12 Issue 2 Pages 135-145  
  Keywords  
  Abstract The soybean cysteine protease inhibitor, soyacystatin N (scN), negatively impacts

growth and development of the cowpea bruchid, Callosobruchus maculatus [Koiwa et

al. (1998) Plant J 14: 371-379]. However, the developmental delay and feeding

inhibition caused by dietary scN occurred only during the early developmental

stages (the 1st, 2nd and 3rd instars) of the cowpea bruchid. The 4th instar

larvae reared on scN diet (adapted) exhibited rates of feeding and development

which were comparable to those feeding on an scN-free diet (unadapted) prior to

pupation. Total gut proteolytic capacity at this larval stage significantly

increased in the scN-adapted insects. The elevated enzymatic activity was

attributed to a differential expression of insect gut cysteine proteases

(representing the major digestive enzymes), and of aspartic proteases. scN

degradation by the gut extract was observed only in adapted bruchids, and this

activity appeared to be a combined effect of scN-induced cysteine and aspartic

proteases. Thirty cDNAs encoding cathepsin L-like cysteine proteases were

isolated from insect guts, and they were differentially regulated by dietary scN.

Our results suggest that the cowpea bruchid adapts to the challenge of scN by

qualitative and quantitative remodelling of its digestive protease complement,

and by activating scN-degrading protease activity.		  
  Address Department of Entomology, Texas A&M University, College Station, TX 77843, USA.  
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  Series Volume Series Issue Edition  
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  Area Expedition Conference  
  Notes Approved no  
  Call Number CSU Fresno @ umuller @ Serial 32  
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