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Author (up) Moon, J.; Salzman, R.A.; Ahn, J.-E.; Koiwa, H.; Zhu-Salzman, K. url  doi
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  Title Transcriptional regulation in cowpea bruchid guts during adaptation to a plant defence protease inhibitor Type Journal Article
  Year 2004 Publication Insect Molecular Biology Abbreviated Journal Insect Mol Biol  
  Volume 13 Issue 3 Pages 283-291  
  Keywords *Adaptation, Physiological; Amino Acid Sequence; Animals; Base Sequence; Beetles/genetics/*metabolism; Blotting, Northern; Cathepsin B/genetics; Cystatins/*metabolism; Cysteine Proteinase Inhibitors/*metabolism; DNA, Complementary/genetics; Digestive System/*metabolism; Gene Expression Profiling; *Gene Expression Regulation; Larva/metabolism; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Sequence Alignment; Sequence Analysis, DNA; Soybean Proteins  
  Abstract Cowpea bruchid, when fed on a diet containing the soybean cysteine protease inhibitor soyacystatin N (scN), activates an array of counter-defence genes to adapt to the negative effects of the inhibitor and regain its normal rate of feeding and development. A collection of 1920 cDNAs was obtained by differential subtraction with cDNAs prepared from guts of the 4th instar larvae of scN-adapted (reared on scN-containing diet) and scN-unadapted (reared on regular scN-free diet) cowpea bruchids. Subsequent expression profiling using DNA microarray and Northern blot analyses identified ninety-four transcript species from this collection that are responsive to dietary scN. scN-adapted insects induced genes encoding protein and carbohydrate digestive enzymes, probably to help meet their carbon and nitrogen requirements. Up-regulation of antimicrobial and detoxification protein genes may represent a generalized defence response. Genes down-regulated by scN reflected physiological adjustments of the cowpea bruchids to scN challenge. A large portion of the responsive genes, presumably involved in carrying out the counter-defence response, were of unknown function. The full-length cDNA of an scN-inducible cathepsin B-like cysteine protease was obtained. Its transcriptional response to scN during larval development contrasts with the pattern of the cathepsin L family, the major digestive enzymes. These results suggest cathepsin B-like cysteine proteases may play a crucial role in cowpea bruchid adaptation to dietary scN.  
  Address Department of Entomology, Texas A&M University, College Station, TX 77843, USA  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0962-1075 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:15157229 Approved no  
  Call Number CUNY @ ffirooznia @ Serial 83  
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