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Author (up) Macedo, M.L.R.; de Castro, M.M.; Freire, M. das G.M. url  doi
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  Title Mechanisms of the insecticidal action of TEL (Talisia esculenta lectin) against Callosobruchus maculatus (Coleoptera: Bruchidae) Type Journal Article
  Year 2004 Publication Archives of Insect Biochemistry and Physiology Abbreviated Journal Arch Insect Biochem Physiol  
  Volume 56 Issue 2 Pages 84-96  
  Keywords Animals; Beetles/*drug effects/enzymology; Biological Assay/methods; Body Weight; Carbohydrates/pharmacology; Cysteine Endopeptidases/metabolism; Digestive System/enzymology; Insecticides/antagonists & inhibitors/metabolism/*pharmacology; Intestinal Mucosa/metabolism; Larva/drug effects; Plant Lectins/antagonists & inhibitors/metabolism/*pharmacology; Sapindaceae/*chemistry; Seeds/chemistry; Survival Analysis  
  Abstract Plant lectins have insecticidal activity that is probably mediated through their ability to bind carbohydrates. To examine the influence of sugars on the insecticidal activity of a lectin from Talisia esculenta seeds (TEL), the lectin was mixed with mannose, glucose, or mannose plus glucose. Mannose abolished the insecticidal activity. Affinity chromatography showed that TEL bound to midgut proteins of the insect Callosobruchus maculatus. Immunoblotting showed that TEL recognized some proteins, probably glycoproteins, present in the midgut membrane of this insect. The principal proteases responsible for digestive proteolysis in fourth instar larvae of C. maculatus were purified by chromatography on activated thiol-Sepharose. These purified proteases were unable to digest TEL after a 15-h incubation. These results suggest that the insecticidal activity of TEL involves a specific carbohydrate-lectin interaction with glycoconjugates on the surface of digestive tract epithelial cells, as well as binding to assimilatory glycoproteins present in midgut extracts and resistance to enzymatic digestion by cysteine proteinases.  
  Address Laboratorio de Purificacao de Proteinas e suas Funcoes Biologicas, Departamento de Ciencias Naturais, Universidade Federal de Mato Grosso do Sul, Tres Lagoas, MS, Brazil  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0739-4462 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:15146543 Approved no  
  Call Number ASU @ Lara.Ferry @ Serial 41  
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