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Author (up) Uchoa, A.F.; DaMatta, R.A.; Retamal, C.A.; Albuquerque-Cunha, J.M.; Souza, S.M.; Samuels, R.I.; Silva, C.P.; Xavier-Filho, J. url  doi
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  Title Presence of the storage seed protein vicilin in internal organs of larval Callosobruchus maculatus (Coleoptera: Bruchidae) Type Journal Article
  Year 2006 Publication Journal of Insect Physiology Abbreviated Journal J Insect Physiol  
  Volume 52 Issue 2 Pages 169-178  
  Keywords Animals; Beetles/*growth & development/metabolism; Blotting, Western; Enzyme-Linked Immunosorbent Assay; Fat Body/metabolism; Hemolymph/metabolism; Immunohistochemistry; Malpighian Tubules/metabolism; Microvilli/metabolism; Plant Proteins/*metabolism; Seed Storage Proteins  
  Abstract Variant vicilins (7S storage globulins) of cowpea seeds (Vigna unguiculata) are considered as the main resistance factor present in some African genotypes against the bruchid Callosobruchus maculatus. It has been suggested that the toxic properties of vicilins may be related to their recognition and interaction with glycoproteins and other membrane constituents along the digestive tract of the insect. However, the possibility of a systemic effect has not yet been investigated. The objective of this work was to study the fate of 7S storage globulins of V. unguiculata in several organs of larvae of the cowpea weevil C. maculatus. Results demonstrated binding of vicilins to brush border membrane vesicles, suggesting the existence of specific receptors. Vicilins were detected in the haemolymph, in the midgut, and in internal organs, such as fat body and malpighian tubules. There is evidence of accumulation of vicilins in the fat body of both larvae and adults. The absorption of vicilins and their presence in insect tissues parallels classical sequestration of secondary compounds.  
  Address Laboratory of Protein and Peptide Biochemistry, Centre for Biosciences and Biotechnology, Universidade Estadual do Norte Fluminense Darcy Ribeiro, CEP 28013-600, Campos dos Goytacazes, RJ, Brazil  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0022-1910 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:16288905 Approved no  
  Call Number ASU @ Lara.Ferry @ Serial 40  
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