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Author (up) Meireles, E.A.; Carneiro, C.N.B.; DaMatta, R.A.; Samuels, R.I.; Silva, C.P. url  doi
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  Title Digestion of starch granules from maize, potato and wheat by larvae of the the yellow mealworm, Tenebrio molitor and the Mexican bean weevil, Zabrotes subfasciatus Type Journal Article
  Year 2009 Publication Journal of Insect Science (Online) Abbreviated Journal J Insect Sci  
  Volume 9 Issue Pages 43  
  Keywords Animals; Digestion/*physiology; Larva/physiology; Microscopy, Electron, Scanning; Solanum tuberosum/*chemistry; Starch/chemistry/*metabolism; Tenebrio/*physiology; Triticum/chemistry; Weevils/*physiology; Zea mays/chemistry  
  Abstract Scanning electron microscopy images were taken of starch granules from different sources following exposure in vivo and in vitro to gut alpha-amylases isolated from Tenebrio molitor L. (Coleoptera: Tenebrionidae) and Zabrotes subfasciatus Boheman (Coleoptera: Bruchidae). One alpha-amylase was isolated from whole larval midguts of T. molitor using non-denaturing SDS-PAGE, while two other alpha-amylase fractions were isolated from whole larval midguts of Z. subfasciatus using hydrophobic interaction chromatography., Digested starch granules from larvae fed on maize, potato or wheat were isolated from midgut contents. Combinations of starch granules with isolated alpha-amylases from both species showed similar patterns of granule degradation. In vitro enzymatic degradation of maize starch granules by the three different alpha-amylase fractions began by creating small holes and crater-like areas on the surface of the granules. Over time, these holes increased in number and area resulting in extensive degradation of the granule structure. Granules from potato did not show formation of pits and craters on their surface, but presented extensive erosion in their interior. For all types of starch, as soon as the interior of the starch granule was reached, the inner layers of amylose and amylopectin were differentially hydrolyzed, resulting in a striated pattern. These data support the hypothesis that the pattern of starch degradation depends more on the granule type than on the alpha-amylase involved.  
  Address Laboratorio de Quimnica e Funcao de Proteinas e Peptideos, Universidade Estadual do Norte Fluminense Darcy Ribeiro, CEP 28013-620, Campos dos Goytacazes, RJ, Brasil  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1536-2442 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:19619014 Approved no  
  Call Number ASU @ Lara.Ferry @ Serial 39  
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