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Author (up) Silva CP, Terra WR, Xavier-Filho J, Grossi de Sa MF, Isejami EM, DeMatta RA, Miguens RA, Bifano TD openurl 
  Title Digestion of legume starch granules by larvae of Zabrotes subfasciatus (Coleoptra: bruchidae) and the induction of alpha-amylases in response to different diets Type Journal Article
  Year 2001 Publication Insect Biochemistry and Molecular Biology Abbreviated Journal Insect Biochem. Mol. Biol.  
  Volume 31 Issue 1 Pages 41-50  
  Keywords  
  Abstract Zabrotes subfasciatus larvae possess three alpha-amylase isoforms as determined by in gel assays following SDS-PAGE. The two minor isoforms present lower electrophoretic mobility than the major form, and seem to occur as a heterodimer. When developed inside Vigna unguiculata (cowpea) seeds, fourth instar larvae have minor quantities of the slow-migrating forms, but when reared on seeds of Phaseolus vulgaris (common bean) or Phaseolus lunatus, the two slow-migrating forms are expressed in higher amounts, while activity of the major form was independent of the host seed. Larvae developing inside cowpea seeds at the beginning of the fourth instar were fed on flour from cotyledons of cowpea or common bean. Larvae fed on the common bean flour started to express the dimer in higher amounts when compared with the control larvae fed on cowpea flour. In an attempt to correlate differences between starch granules and the induction of alpha-amylases, a detailed study on the digestive process of the granules was conducted. Incorporation of purified starch granules into artificial diets did not induce the two minor alpha-amylases. The in vitro hydrolysis rates of purified granules and the pattern of dextrins liberated by the different alpha-amylases were similar for the two legume species. The starch granules enter the midgut extensively damaged, which may facilitate the access to the more susceptible parts of the granules to enzymatic attack.  
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  Call Number Roanoke @ watkinson @ Serial 33  
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