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Author (up) Ximenes, A.A.; Oliveira, G.A.; Bittencourt-Cunha, P.; Tomokyo, M.; Leite, D.B.; Folly, E.; Golodne, D.M.; Atella, G.C. url  openurl
  Title Purification, partial characterization and role in lipid transport to developing oocytes of a novel lipophorin from the cowpea weevil, Callosobruchus maculatus Type Journal Article
  Year 2008 Publication Brazilian Journal of Medical and Biological Research Abbreviated Journal Braz J Med Biol Res  
  Volume 41 Issue 1 Pages 18-25  
  Keywords Animals; Apolipoproteins/chemistry/isolation & purification/metabolism; Biological Transport; Endocytosis/physiology; Female; Hydrocarbons/*analysis; Lipid Metabolism/*physiology; Lipoproteins/*chemistry/isolation & purification/metabolism; Oocytes/*growth & development/metabolism; Oogenesis/physiology; Phospholipids/*chemistry/isolation & purification/metabolism; Weevils/*chemistry/metabolism  
  Abstract Lipid transport in arthropods is achieved by highly specialized lipoproteins, which resemble those described in vertebrate blood. Here we describe purification and characterization of the lipid-apolipoprotein complex, lipophorin (Lp), from adults and larvae of the cowpea weevil Callosobruchus maculatus. We also describe the Lp-mediated lipid transfer to developing oocytes. Lps were isolated from homogenates of C. maculatus larvae and adults by potassio bromide gradient and characterized with respect to physicochemical properties and lipid content. The weevil Lp (465 kDa) and larval Lp (585 kDa), with hydrated densities of 1.22 and 1.14 g/mL, contained 34 and 56% lipids and 9 and 7% carbohydrates, respectively. In both Lps, mannose was the predominant monosaccharide detected by paper chromatography. SDS-PAGE revealed two apolipoproteins in each Lp with molecular masses of 225 kDa (apolipoprotein-I) and 79 kDa (apolipoprotein-II). The lipids were extracted and analyzed by thin-layer chromatography. The major phospholipids found were phosphatidylserine, phosphatidylcholine and phosphatidylethanolamine in adult Lp, and phosphatidylcholine, phosphatidylethanolamine and sphingomyelin in larval Lp. Hydrocarbons, fatty acids and triacylglycerol were the major neutral lipids found in both Lps. Lps labeled in the protein moiety with radioactive iodine (125I-iodine) or in the lipid moiety with fluorescent lipids revealed direct evidence of endocytic uptake of Lps in live oocytes of C. maculatus.  
  Address Instituto de Bioquimica Medica, Centro de Ciencias da Saude, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0100-879X ISBN Medium  
  Area Expedition Conference  
  Notes PMID:18038102 Approved no  
  Call Number WNEC @ jrocheleau @ Serial 32  
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