Bean Beetle Home Page Home

Bean Beetle Literature Database for Ecology, Evolution, and Animal Behavior

Bean Beetle Home Page |  Bibliography Home |  Show All |  Simple Search |  Advanced Search
Quick Search:
1-1 of 1 record found matching your query:
toggle visibility Search & Display Options
Select All    Deselect All << 1 >>
List View
 | 
Citations
 | 
Details
   print
  Record Links
Author (up) Souza, S.M.; Uchoa, A.F.; Silva, J.R.; Samuels, R.I.; Oliveira, A.E.A.; Oliveira, E.M.; Linhares, R.T.; Alexandre, D.; Silva, C.P. url  doi
openurl 
  Title The fate of vicilins, 7S storage globulins, in larvae and adult Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchinae) Type Journal Article
  Year 2010 Publication Journal of Insect Physiology Abbreviated Journal J Insect Physiol  
  Volume 56 Issue 9 Pages 1130-1138  
  Keywords Analysis of Variance; Animals; Beetles/*metabolism; Blotting, Western; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Fabaceae/*chemistry; Fat Body/metabolism; Female; Fluorescein-5-isothiocyanate; Larva/metabolism; Male; Oocytes/metabolism; Ovum/metabolism; Seed Storage Proteins/blood/*metabolism  
  Abstract The fate of vicilins ingested by Callosobruchus maculatus and the physiological importance of these proteins in larvae and adults were investigated. Vicilins were quantified by ELISA in the haemolymph and fat body during larval development (2nd to 4th instars), in pupae and adults, as well as in ovaries and eggs. Western blot analysis demonstrated that the majority of absorbed vicilins were degraded in the fat body. Tracing the fate of vicilins using FITC revealed that the FITC-vicilin complex was present inside cells of the fat body of the larvae and in the fat bodies of both male and female adult C. maculatus. Labelled vicilin was also detected in ovocytes and eggs. Based on the results presented here, we propose that following absorption, vicilins accumulate in the fat body, where they are partially degraded. These peptides are retained throughout the development of the insects and eventually are sequestered by the eggs. It is possible that accumulation in the eggs is a defensive strategy against pathogen attack as these peptides are known to have antimicrobial activity. Quantifications performed on internal organs from larvae of C. maculatus exposed to extremely dry seeds demonstrated that the vicilin concentration in the haemolymph and fat body was significantly higher when compared to larvae fed on control seeds. These results suggest that absorbed vicilins may also be involved in the survival of larvae in dry environments.  
  Address Laboratorio de Quimica e Funcao de Proteinas e Peptideos, Centro de Biociencias e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brazil  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0022-1910 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:20230826 Approved no  
  Call Number North Georgia @ srsonger @ Serial 16  
Permanent link to this record
Select All    Deselect All << 1 >>
List View
 | 
Citations
 | 
Details
   print
toggle visibility
Save Citations:
Export Records:
Home Library Search  |  Show Record  |  Extract Citations
This website is supported by National Science Foundation Grants, DUE-0535903, DUE-0815135, DUE-0814373, DUE-1821533, and DUE-1821184 to Morehouse College and Emory University, Atlanta, Georgia, USA. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessary reflect the views of the National Science Foundation, Emory University, or Morehouse College. Copyright © by Lawrence S. Blumer and Christopher W. Beck, 2008-2014. All rights reserved. The content of this site may be freely used for non-profit educational purposes, with proper acknowledgement of the source. All other uses are prohibited without prior written permission from the copyright holders.