These data were collected by Dwann Davenport at Morehouse College in Spring 2024. Beetles were offspring reared on blackeye peas(N=6)and adzuki beans (N=7)whose mothers host bean was blackeye peas. Data set also includes one negative control. Beetles were from the SI-3 strain adapted to their host bean for many generations prior to eggs laid on either blackeye peas or adzuki beans. Beetles were adult of unknown sex. Beetles were surface-sterilized. Beetles were extracted with QIAGEN DNA-Easy kits. Sequences are for the v4 region. MiSeq sequencing was done by MrDNA labs. Metadata and Sequence Sample ID notation is "Mothers host.Offspring host". For more information, contact Dwann Davenport .