These data were collected by students at Fayetteville State University in Fall 2022. Beetles were reared on black-eyed peas. Beetles were from the LB strain adapted to their host bean for many generations. Beetles were females. Beetles were adults. Beetles were surface-sterilized. Half of the beetles were extracted using the standard extraction with QIAGEN DNA-Easy kits. The other half of the beetles were extracted using the Dneasy Gram + Protocol Using Enzymatic Lysis Buffer Including Lysozyme Sequences are for the v4 region. MiSeq sequencing was done by MrDNA labs. For more information, contact Kristen Delaney Nguyen at kdelaney@uncfsu.edu.