Half-semester Curriculum

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Manipulative Experiment

WeekActivityResources
1Lab safety, Introduction to Microbiomes, Introduction to Bean Beetles, Experimental Design, Experimental Manipulation, Pre-semester Survey
2Other curriculum to allow for beetle development
3Other curriculum
4Other curriculum
5Other curriculum
6Initial steps of DNA extraction for microbial community sequencing, culturing microbes
7Phenotypic assessment of microbes, colony-based PCR
8Electrophoresis and Iteration Day for failed culture-based PCR
9Introduction to bioinformatics & analysis of colony sequencing data, introduction to community analysis, phenotype & colony sequencing analysis
10Other curriculum to allow for DNA sequencing to be completed
11Other curriculum
12Other curriculum
13Community analysis of sequencing data

  • BeanBeetleMicrobiome app Tutorial [docx] [pdf]

14Final presentations, Post-semester survey